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- 技术资料
- 抗体名:
Brilliant Violet 421™ anti-human CD117 (c-kit)
- 抗体英文名:
Brilliant Violet 421™ anti-human CD117 (c-kit)
- 应用范围:
免疫荧光,流式
- 宿主:
小鼠
- 供应商:
上海善然生物
- 库存:
现货
- 抗原来源:
小鼠
- 保质期:
2-3年左右
- 适应物种:
Human Reported Reactivity Cynomolgus, Cow
- 标记物:
Brilliant Violet 421™
- 克隆性:
单克隆
- 保存条件:
2-8度
- 形态:
液体
- 规格:
100 μg
Brilliant Violet 421™ anti-human CD117 (c-kit) Antibody
104D2
Catalog# / Size 313215 / 25 tests
313216 / 100 tests
Clone
Regulatory Status RUO
Other Names Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype Mouse IgG1, κ
Description CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell
factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells
(approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML).
CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation
and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulomonocytic committed cells.
Product Details
Verified Reactivity Human
Reported Reactivity Cynomolgus, Cow
Antibody Type Monoclonal
Host Species Mouse
Immunogen MOLM-1 megakaryocytic cell line
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™
under optimal conditions.
Concentration Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our
Certificate of Analysis online tool.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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Vybrant® DyeCycle Violet Stain
Fluor® 488 and PE channels Eliminate or correct for cell aggregates during data analysis using gating or modeling software Human and rodent stem cells efflux Vybrant® DyeCycle™ Violet stain, and this is the basis for the Side Population
LIVE/DEAD® Violet Viability/Vitality Kit
. Material Amount Storage Stability CellTrace™ calcein violet stain, lyophilized (Component A) *
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
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