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- DIMA BIOTECH/缔码生物
- 武汉
- PME101225
- 2026年07月10日
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- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期：
12个月
- 英文名：
Recombinant Human AZU1 Protein with C-terminal 6×His tag
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
10μg
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | AZU1 |
| Uniprot ID | P20160 |
| 背景 | Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. This gene encodes a preproprotein that is proteolytically processed to generate a mature azurophil granule antibiotic protein, with monocyte chemotactic and antimicrobial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Nov 2015] |
| 货期 | In Stock |
| 产品英文名称 | Recombinant Human AZU1 Protein with C-terminal 6×His tag |
| 分子结构 | AZU1(Ile27-Pro248) 6×His tag |
| 分子量 | The protein has a predicted molecular mass of 24.9 kDa after removal of the signal peptide. The apparent molecular mass of AZU1-His is approximately 35-55 kDa due to glycosylation. |
| 纯度 | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | AZAMP;AZU;CAP37;HBP;hHBP;HUMAZUR;NAZC |
| 标签 | C-6×His Tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 应用范围 | Research use only |
Figure 1. Human AZU1 Protein, His Tag on SDS-PAGE under reducing condition.风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L 100mM Imidizole 3.4g 5% glycerol 25ml 100% glycerol 50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9) 0.1% Tween-20 0.5M 100% Tween-20 500mM NaCl50ml 5M NaCl dH2O Fill to 0.5L (start w/ 350ml) High (“High
噬菌体展示技术是一种高效基因表达筛选技术，将外源蛋白或多肽与特定的噬菌体衣壳蛋白融合，展示在噬菌体表面并保持相对独立的空间构象和生物活性，有利于靶分子的特异性识别及结合，从而实现基因型和表达型的统一。 噬菌体展示技术因其独特的优势，现已广泛应用于抗原表位分析、单克隆抗体的制备、抗体人源化、药物筛选、疫苗研制以及免疫学疾病诊断、治疗等科学研究领域。 噬菌体技术为啥这么好用？为了一探究竟，我们先来弄清噬菌体是个啥？ 噬菌体是感染细菌、真菌、放线菌或螺旋体等微生物病毒的总称，因部分能引起
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