- ￥4500 - 5500
- NEWGAINBIO
- IH1020-5
- 江苏
- 2025年11月08日
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
白色
- ATCC Number：
11111
- 细胞类型：
永生化细胞
- 肿瘤类型：
否
- 供应商：
欣润生物
- 库存：
10
- 英文名：
Immortalized human adipose derived mesenchymal stem cells
- 生长状态：
贴壁生长
- 年限：
成年
- 运输方式：
新鲜顺丰运输
- 器官来源：
脂肪
- 是否是肿瘤细胞：
否
- 细胞形态：
成纤维细胞样
- 免疫类型：
正常
- 物种来源：
人
- 相关疾病：
无
- 组织来源：
脂肪组织
- 规格：
T25方瓶
永生化人脂肪间充质干细胞简介：
产品描述：人脂肪间充质干细胞分离自成年人皮下脂肪组织，体外培养的脂肪间充质干细胞呈纤维细胞样生长，为短梭形、小三角形以及多角形等。来源于中胚层的脂肪组织的干细胞具有向成脂肪、成软骨、成骨、心肌细胞和神经源细胞分化的多分化潜能。因此脂肪组织可以作为人间充质干细胞库的重要来源，并可作为多种组织工程的种子细胞，有非常重要的研究和应用价值。
产品货号：IH1020
产品类型: 永生化细胞
传代能力: 30代左右
产品形态: 成纤维细胞形态
培养基：永生化人脂肪间充质干细胞完全培养基，产品编号：IH1020-5,100ml或500ml规格
支原体质控：质控呈现阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子顺丰快递运输
货期：1周左右货期
永生化人脂肪间充质干细胞白光及脂肪诱导分化图
An immortalized human adipose-derived stem cell line with highly enhanced chondrogenic properties
Human adipose-derived stem cells (ASCs) are a commonly used cell type for cartilage tissue engineering. However, donor-to-donor variability, cell heterogeneity, inconsistent chondrogenic potential, and limited expansion potential can hinder the use of these cells for modeling chondrogenesis, in vitro screening of drugs and treatments for joint diseases, or translational applications for tissue engineered cartilage repair. The goal of this study was to create an immortalized ASC line that showed enhanced and consistent chondrogenic potential for applications in cartilage tissue engineering as well as to provide a platform for investigation of biological and mechanobiological pathways involved in cartilage homeostasis and disease. Starting with the ASC52telo cell line, a hTERT-immortalized ASC line, we used lentivirus to overexpress SOX9, a master regulator of chondrogenesis, and screened several clonal populations of SOX9 overexpressing cells to form a new stable cell line with high chondrogenic potential. One clonal line, named ASC52telo-SOX9, displayed increased GAG and type II
Telomerase immortalized human amnion- and adipose-derived mesenchymal stem cells: maintenance of differentiation and immunomodulatory characteristics.
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
