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- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IH1016
- 2026年04月22日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Immortalized Human Urethral Fibroblasts
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
人源
- 组织来源：
尿道
- 相关疾病：
无
- 物种来源：
人源
- 免疫类型：
不详
- 细胞形态：
成纤维细胞样
- 是否是肿瘤细胞：
否
- 器官来源：
尿道
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化人尿道成纤维细胞
细胞简介：
虽然原代人尿道成纤维细胞更能真实地反映尿道成纤维细胞在人体内的生理状态，但是一方面人体尿道组织非常珍贵，原代分离培养人尿道成纤维细胞周期长及对技术人员经验要求较高，另一方面此原代细胞生长较慢及在体外不能多次传代，这些不利因素制约了原代人尿道成纤维细胞在实验室的广泛应用。我们的研究团队拥有多年原代细胞分离培养及细胞永生化服务研究经验，成功建立了永生化人尿道成纤维细胞。
本公司生产的永生化人尿道成纤维细胞采用酶解法和基因转染制备而来，细胞总量约为1×106/T25方瓶，细胞纯度可达90%以上，且不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
培养基信息：
我们推荐使用我们的研制的永生化人尿道成纤维细胞完全培养基（产品编号：IH1016-5）作为体外培养永生化人尿道成纤维细胞的培养基。
Molecular characterization of human telomerase reverse transcriptase-immortalized human fibroblasts by gene expression profiling: activation of the epiregulin gene.
Reconstitution of telomerase activity by ectopic expression of telomerase reverse transcriptase (hTERT) results in an immortal phenotype in various types of normal human cells, including fibroblasts. Despite lack of transformation characteristics, it is unclear whether hTERT-immortalized cells are physiologically and biochemically the same as their normal counterparts. Here, we compared the gene expression profiles of normal and hTERT-immortalized fibroblasts by using a cDNA microarray containing 20,736 cDNA clones and identified 172 dysregulated genes or expressed sequence tags (ESTs). One of the highly expressed genes in the hTERT-immortalized fibroblasts (hTERT-BJ cells) encodes epiregulin, a potent growth factor. Blockade of epiregulin reduced the growth of hTERT-BJ cells and colony formation of hTERT-transformed fibroblasts. Moreover, inhibition of epiregulin function in immortal hTERT-BJ cells triggered a senescence program. Our results suggest that both activation of telomerase and subsequent induction of epiregulin are required for sustained
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- 作者
- 内容
- 询问日期
文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
为同一类型的细胞，如上皮细胞或成纤维细胞，也仍具有异质性，在分析细胞生物学特性时比较困难。其次，由于供体的个体差异及其他一些原因，细胞群生长效果有时也不一致。原代培养也是建立各种细胞系（株）必经的阶段。假如原代培养能够维持几小时甚至更长，即可进行进一步筛选。有的细胞具有继续增殖能力，有的细胞类型只是存活而不增殖，而另外一些细胞只是在特殊条件下应用而不存活，因而细胞类型的分布将会改变。在单层培养的情况下，瓶底全部铺满细胞，达到会合以后，对密度有依赖性的细胞则逐渐减少生长，而失去密度依赖敏感性的细胞
基质上培养 (贴壁或者单层培养)，而另一些细胞则可在培养基中漂浮生长 (悬浮培养)。冻存如果传代时有多余的细胞，可通过适当的保护剂 (例如：DMSO 或甘油) 处理细胞，储存在 –130°C 以下的温度下 (冻存)，直到需要使用之时。 培养细胞的形态 根据形状和外观 (即：形态) 可将培养的细胞分为三大类。 • 成纤维细胞 (或者成纤维细胞样细胞) 为双极或多极细胞，呈细长形，贴附在基质上 生长。 • 上皮样细胞呈多角形，尺寸更为规则，贴附在基质上呈散在斑
基因编辑再次升级！领域大牛刘如谦 Cell 发文开发新工具，可安全高效进行体内基因编辑
4 BE-eVLPs 支持有效、高效的基因编辑，并且显示出最小的脱靶编辑效率和 DNA 整合风险。 图片来源：Cell v4 BE-eVLPs 有效地编辑人类原代细胞和小鼠细胞 为了进一步探索 v4 BE-eVLPs 的效用，他们评估了其在体外靶向和编辑多种原代人类或小鼠细胞的能力。在转导含有纯合子 COL7A1（R185X）突变的原代人类成纤维细胞中，他们观察到高于 95% 的修复效率。这种高效率在小鼠模型的原代成纤维细胞中也得到了重现。 这些结果验证了 v4 BE-eVLPs 的活性并不局限于细胞系
技术资料
