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- 欣润生物
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- IP4004-1
- 2025年12月14日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Porcine small intestine smooth muscle cell line
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
NO
- 细胞类型：
永生化
- ATCC Number：
11222
- 品系：
巴马猪
- 组织来源：
小肠组织
- 相关疾病：
无
- 物种来源：
猪
- 免疫类型：
不详
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
小肠
- 运输方式：
常温
- 年限：
新生
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化猪小肠平滑肌细胞简介：
产品描述：猪小肠平滑肌细胞分离自猪小肠组织。小肠位于腹中，上端接幽门与胃相通，下端通过阑门与大肠相连，是食物消化吸收的主要场所。小肠有三种功能即消化、吸收和分泌及运动功能，其中以吸收和分泌功能为主。平滑肌细胞的收缩是负责肠蠕动的动力，促使食物向下运动。
产品货号：IP4004
产品类型: 永生化细胞
传代能力: 30代左右
产品形态: 梭形
培养基：永生化猪小肠平滑肌细胞完全培养基
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：1周左右货期
a-SMA抗体免疫荧光染色鉴定
Gene expression and activity of enzymes in the arginine biosynthetic pathway in porcine fetal small intestine
We recently reported the presence of ornithine aminotransferase (OAT) enzymatic activity and mRNA expression in the intestine of fetal pigs from 30 to 110 d of gestation. Here we describe the activities and mRNA expression patterns of other key enzymes in the arginine biosynthetic pathway, specifically carbamoyl phosphate synthase I (CPS-I), ornithine carbamoyl transferase (OCT), and pyrroline-5-carboxylate reductase (P5CR), in the fetal porcine small intestine from 30 to 110 d of gestation. The activities of all three enzymes increased from d 30 to d 110 of gestation, and in situ hybridization demonstrates that 1) CPS-I and OCT genes are expressed in distinct patterns and are confined to the mucosal epithelium and 2) P5CR mRNA is present in mucosal epithelium and lamina propria of the fetal porcine small intestine from d 30 to d 110 of gestation. The presence of CPS-I and OCT in conjunction with the presence of OAT suggests that the fetal porcine small intestine is capable of synthesizing citrulline from P5C. In addition, the presence of P5CR suggests that the fetal porcine small intestine is
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
主要有两类：神经嵴干细胞(neuralcreststemcell，NC-SC)和中枢神经干细胞(CNS-SC)。NCSC为外周神经干细胞(PNS-SC)，既可发育为外周神经细胞、神经内分泌细胞和Schwann氏细胞，也能分化为色素细胞(pigmented cell)和平滑肌细胞等。NSC一般是指存在于脑部的中枢神经干细胞(CNS-SC)，其子代细胞能分化成为神经系统的大部分细胞。以往认为，中枢神经系统的神经元在出生前或出生后不久，就失去再生能力。但近年的一些研究表明，成年哺乳
、物理、病毒等方法）诱导细胞发生转化，使其倍增时间减少，永生化或生命期延长，也是一个努力方向。 但是要建立适于组织工程需要的种子细胞，需要解决以下问题：①增加细胞的增殖能力；②延长细胞的生命期；③提高细胞的分泌能力；④优选不同组织来源的同一功能的最佳细胞；⑤建立标准细胞系，使研究工作有更好的可比性和科学性；⑥同种异体与异种移植的免疫学；⑦细胞与人工细胞外基质的相互作用及影响因素。 采用同种异体细胞来源，在目前仅对少数组织细胞（如软骨组织的组织工程培养）有望获得
LFA-3（IGSF） LFA-3（CD58） 广泛 40～65 LFA-2（IGSF） ICAM-1（CD54） 白细胞、内皮细胞、某些肿瘤细胞、上皮细胞、肝细胞、平滑肌细胞 80～114 LFA-1（粘合素超家族） ICAM-2（CD102） 内皮细胞 60 LFA-1（粘合素超家族） ICAM-3（CD50） T细胞、单核细胞、中性粒细胞 140/108 CD4 抑制细胞诱导亚群、辅助细胞诱导亚群 55 MHC-Ⅱ(IGSF) CD8 抑制性T细胞/杀伤性T细胞 34/34 MHC-Ⅰ
技术资料