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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
(MOPC-21) Mouse APC SNFA-685 PerCP Mouse IgG1,κ Isotype Control(MOPC-21) Mouse PerCP SNFA-399 FITC Mouse IgG2a,κ Isotype Control(C1.18.4) Mouse FITC SNFA-559 PE Mouse IgG2a,κ Isotype Control(C1.18.4) Mouse PE SNFA
Immunofluorescent Staining of Mouse and Rat Leukocytes
. Examples of negative controls are unstained cells, cells exposed to the 2nd step reagent alone, or cells exposed to isotype controls which are the same isotype and format (e.g.,purified, biotin or fluorochrome) as the primary antibody and titrated
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