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- FHTDM-1 (for 1ml)
- 2026年01月10日
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DNA-Free HS Taq-D 2×SuperMix contains hot-start Taq-D DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. For DNA amplification, simply add the template, primers, and water to adjust the 2× SuperMix solution to a 1× concentration to proceed with the reaction. The amplification products have an "A" base at the 3' end, making them directly suitable for gene cloning. DNA-Free HS Taq-D 2× SuperMix (dye) contains electrophoresis dye, allowing amplification products to be directly loaded for electrophoresis. If used for cloning, the dye needs to be purified away.
Cat. No.: FHTDM-1 (for 1ml)
Cat. No.: FHTDM-5 (for 5ml)
Cat. No.: FHTDM-20 (for 20ml)
Cat. No.: FHTDM-50 (for 50ml)
Description
DNA-Free HS Taq-D 2×SuperMix contains hot-start Taq-D DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. For DNA amplification, simply add the template, primers, and water to adjust the 2× SuperMix solution to a 1× concentration to proceed with the reaction. The amplification products have an "A" base at the 3' end, making them directly suitable for gene cloning. DNA-Free HS Taq-D 2× SuperMix (dye) contains electrophoresis dye, allowing amplification products to be directly loaded for electrophoresis. If used for cloning, the dye needs to be purified away.
Features
- Completely free from bacterial DNA.
- High amplification efficiency.
- Hot-start functionality.
- High product specificity.
- Good stability.
Applications
- Hot-start standard PCR.
- Direct PCR from tissue and blood samples.
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文献和实验。 a.时间越长，DNA的产量越多，污染也越多 7. 12000转速离心5-10min，去上清，倒置于吸水纸上数分钟，加入700µl 70%的酒精，上下颠倒数次，洗涤沉淀。12000转离心5-10m，去上清，倒置于吸水纸上数分钟。重复一遍。 8. 12000转速离心5-10min，去上清，倒置于吸水纸上数分钟，最后置于超净工作台中吹干。 9. 在离心管中加入30-50µl的灭菌去离子水或者TE溶液，4℃放置数小时，使其充分溶解。 10
指DNA双链的局部，由具有互补性单链 DNA与之结合所产生的环状结构。当 DNA复制开始，在原来的双链中仅一方被新合成的短 DNA单链被置换的情况下可以见到（为 Displacement loop之简称）。可通过人工使 DNA单链结合来制成此结构。由 RNA单链所产生的类似结构称为 R环。
Assay of Phospholipase D Activity in Cell-Free Systems
Phospholipase D (PLD) enzymes are present in all animal and plant species and have been linked to many critical cellular processes, including proliferation, differentiation, motility, and secretion. The functional significance of PLD derives
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