DNA-Free Taq-D DNA Polymerase

Taq-D DNA Polymerase is a direct-acting Taq DNA polymerase, which is a modified form of Taq DNA polymerase derived from the thermophilic bacterium Thermus aquaticus, recombinantly expressed in Escherichia coli. This enzyme has been genetically engineered and is particularly suitable for amplifying samples containing PCR inhibitors, such as blood. It can amplify DNA fragments up to 3 kb in length. The extension rate is 1.0 kb/min at 72°C. This enzyme exhibits 5'→3' polymerase activity, without 5'→3' exonuclease activity or 3'→5' exonuclease activity. The amplified products have 3'-dA tails. This enzyme is not suitable for Taqman probe-based q-PCR. It is particularly well-suited for single nucleotide polymorphism (SNP) genotyping using the melting curve method.

Cat. No.: DFEDUT-500 (for 500U)

Cat. No.: DFEDUT-2500 (for 500U×5)

Cat. No.: DFEDUT-10KU (for 2500U×4)

Cat. No.: DFEDUT-25KU (for 2500U×10)

 

The lyophilized Taq-D DNA Polymerase is also available by inquiry, which can be transported at room temperature.

 

Description

Taq-D DNA Polymerase is a direct-acting Taq DNA polymerase, which is a modified form of Taq DNA polymerase derived from the thermophilic bacterium Thermus aquaticus, recombinantly expressed in Escherichia coli. This enzyme has been genetically engineered and is particularly suitable for amplifying samples containing PCR inhibitors, such as blood. It can amplify DNA fragments up to 3 kb in length. The extension rate is 1.0 kb/min at 72°C. This enzyme exhibits 5'→3' polymerase activity, without 5'→3' exonuclease activity or 3'→5' exonuclease activity. The amplified products have 3'-dA tails. This enzyme is not suitable for Taqman probe-based q-PCR. It is particularly well-suited for single nucleotide polymorphism (SNP) genotyping using the melting curve method.

Our DNA-Free Taq-D DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes Taq-D DNA Polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

 

Activity Definition

The enzyme activity is defined as the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotides into acid-insoluble material at 72°C for 30 minutes, using Mahi Mahi sperm DNA as a template/primer, and is expressed as one unit (U).

 

Concentration

5 U/μl

 

Features

• No exonuclease activity, suitable for single nucleotide polymorphism (SNP) genotyping using the melting curve method.
• Excellent heat stability: Half-life exceeds 40 minutes at 95°C.
• Can incorporate dUTP, dITP, and fluorescently labeled nucleotides.
• Compared to regular Taq DNA polymerase, this enzyme is more suitable for PCR with untreated blood, tissue, saliva, and other samples.

 

Applications

• Melting curve-based gene typing/SNP analysis.
• Direct PCR with blood, tissue samples, and more.
• DNA fluorescent labeling.
• Colony PCR.
• Addition of 3’-dA to TA cloning PCR products.

 

Storage

 

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

 

Instruction: Protocol

Related: U-Taq DNA Polymerase (Glycerol Free), 2 x Taq PCR MasterMix, Pfu DNA Polymerase, Taq Monoclonal Antibody