DNA-Free热启动高保真Taq DNA聚合酶

DNA-Free热启动高保真Taq DNA聚合酶需要通过95℃加热5分钟或98℃加热2分钟激活才能发挥最佳活性。这款创新型PCR酶制剂基于Barnes方法开发，通过将具有强大3'→5'外切核酸酶校对活性的KOD DNA聚合酶与Taq DNA聚合酶进行精确配比复合，不仅显著提升了扩增效率，更将保真度推向新高度。其热启动特性可有效抑制非特异性扩增和引物二聚体形成，特别适合复杂模板的精准扩增。经过特殊改造的酶组分展现出卓越性能：能高效扩增长达5-6kb的DNA片段，在72℃条件下延伸速度达1-2kb/分钟，同时具备5'→3'聚合酶活性、弱5'→3'外切活性和优化的3'→5'校对活性，扩增产物部分带有3'-dA突出末端。采用专利纯化工艺彻底清除生产菌株的基因组和质粒DNA污染，使其成为16S/23S rRNA基因研究的金标准，特别适用于高精度扩增、测序与克隆，以及对扩增特异性要求严苛的分子实验，同时也能完美胜任常规PCR及引物延伸等标准实验，为研究人员提供了兼具超高灵敏度、卓越特异性和保真度的PCR解决方案。

Cat. No.: DFHSFT-250 (for 250U)

Cat. No.: DFHSFT-1250 (for 1250U)

Cat. No.: DFHSFT-5k (for 5KU)

Cat. No.: DFHSFT-12500 (for 12500U)

Description

DNA-Free HS HiFi Taq DNA Polymerase is the hot-start version of HiFi Taq DNA Polymerase, requiring activation by heating at 95°C for 5 minutes or at 98°C for 2 minutes. It enhances amplification specificity and reduces non-specific amplification and primer-dimer formation. HiFi Taq DNA Polymerase is a high-efficiency PCR enzyme developed based on the Barnes method. It combines KOD DNA polymerase, with strong 3'→5' exonuclease activity (proofreading activity), and Taq DNA polymerase in an optimal ratio, achieving superior PCR performance and improved fidelity. Both enzymes are specially modified for enhanced amplification capability and extension speed, with fragment lengths up to 5-6 kb and an extension rate of 1-2 kb/min (72°C). The enzyme exhibits 5'→3' polymerase activity, weak 5'→3' exonuclease activity, and optimized 3'→5' exonuclease activity. A small amount of amplification product has 3'-dA overhangs.

Our DNA-Free HS HiFi Taq DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes this polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Features

• Completely free from bacterial DNA.

• Specially modified for enhanced amplification capability, effectively amplifying 3-5kb DNA fragments at a rate of 2kb/min.

• Enhances amplification specificity, reducing non-specific amplification and primer-dimer formation.

• Excellent thermal stability: Half-life exceeds 40 minutes at 95°C.

• A small amount of PCR product has 3'-dA overhangs, making direct T/A cloning slightly less efficient.

• Broad tolerance range for template DNA.

Applications

• Conventional PCR amplification

• Colony PCR

• Multiplex PCR

• DNA fluorescence labeling

Unit Definition

The amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acid-insoluble material within 30 minutes at 72°C, using salmon sperm DNA as the template/primer, is defined as one activity unit (U).

Storage

The minimum shelf life is a year at -20°C. Avoid repeated freeze-thaw cycles.