- ￥1120
- SBS
- DFRNT-200 (for 1200U)
- 2026年04月14日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
DNA-Free RNase T, also known as Exonuclease T (Exo T), is a nuclease specific for single-stranded RNA or DNA. This enzyme requires a free 3' end and removes nucleotides in the 3' to 5' direction. RNase T exhibits ten times higher activity on DNA than RNA and can be used to blunt the ends of RNA or DNA molecules with 3' overhangs. It is a mesophilic enzyme and can be inactivated by incubation at 65℃ for 20 minutes. Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.
Cat. No.: DFRNT-200 (for 1200U)
Cat. No.: DFRNT-1k (for 1000U)
Description
DNA-Free RNase T, also known as Exonuclease T (Exo T), is a nuclease specific for single-stranded RNA or DNA. This enzyme requires a free 3' end and removes nucleotides in the 3' to 5' direction. RNase T exhibits ten times higher activity on DNA than RNA and can be used to blunt the ends of RNA or DNA molecules with 3' overhangs. It is a mesophilic enzyme and can be inactivated by incubation at 65℃ for 20 minutes.
Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.
Unit Definition
One unit is defined as the amount of enzyme required to hydrolyze 1 nmol of [3H]-labeled poly(thymidylic acid) to 0.1 nmol of trichloroacetic acid-soluble nucleotides in 30 minutes at 25℃ in a 1× AM Buffer D reaction system.
Features
- Completely free from bacterial DNA.
- Removes 3' overhangs of double-stranded DNA, creating blunt ends.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
Preparation of RNase-Free DNase by Alkylation
Characterization of RNA molecules by electrophoresis or hybridization frequently requires nucleic acid concentrations over 1 mg/mL. High molecular weight DNA in a mixture of nucleic acids limits the solubility and interferes
RNase A/T1 mapping provides a sensitive and quantitative method of expression analysis of known gene sequences. It differs from primer-derived analyses such as primer extension and reverse transcriptase-PCR by the use of a probe
技术资料暂无技术资料 索取技术资料
