DNA-Free Taq DNA Polymerase

DNA-Free Taq DNA Polymerase is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free Taq particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Cat. No.: DFTDP-500 (for 500U)

Cat. No.: DFTDP-2500 (for 2500U)

Cat. No.: DFTDP-10k (for 10KU)

Cat. No.: DFTDP-25k (for 25KU)

Description

Taq DNA Polymerase, initially derived from Thermus aquaticus, is commonly produced from its cloned gene expressed in E. coli for large-scale manufacturing. However, Taq polymerase sourced from E. coli often contains residual bacterial DNA, likely originating from the expression vector system and other manufacturing processes. This contamination can restrict the use of Taq DNA Polymerase in PCR reactions for certain samples. Some reports suggest that commercially available Taq DNA Polymerase may contain up to 1,000 genome equivalents of bacterial DNA per unit of enzyme.

Our DNA-Free Taq DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free Taq particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Features

• Completely free from bacterial DNA.
• Versatile buffer system suitable for all applications.
• High sensitivity, enabling efficient amplification genes.
• High productivity, ensuring high yields of products.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

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DNA-Free Pfu DNA Polymerase