- ￥336
- SBS
- FHTMD-1 (for 1ml)
- 2026年01月10日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
DNA-Free HS Taq 2×SuperMix contains a DNA-Free HS Taq DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. For DNA amplification, simply add the template, primers, and water to adjust the 2× SuperMix solution to a 1× concentration to proceed with the reaction. The amplification products have an "A" base at the 3' end, making them directly suitable for gene cloning. DNA-Free HS Taq 2× SuperMix (dye) contains electrophoresis dye, allowing amplification products to be directly loaded for electrophoresis. If used for cloning, the dye needs to be purified away.
Cat. No.: FHTMD-1 (for 1ml)
Cat. No.: FHTMD-5 (for 5ml)
Cat. No.: FHTMD-20 (for 20ml)
Cat. No.: FHTMD-50 (for 50ml)
Description
DNA-Free HS Taq 2×SuperMix contains a DNA-Free HS Taq DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. For DNA amplification, simply add the template, primers, and water to adjust the 2× SuperMix solution to a 1× concentration to proceed with the reaction. The amplification products have an "A" base at the 3' end, making them directly suitable for gene cloning. DNA-Free HS Taq 2× SuperMix (dye) contains electrophoresis dye, allowing amplification products to be directly loaded for electrophoresis. If used for cloning, the dye needs to be purified away.
Features
- Completely free from bacterial DNA.
- Reduces PCR amplification time.
- Prevents contamination from multiple steps.
- Suitable for amplifying genomic DNA fragments (≤4 kb).
Applications
- Hot-start PCR amplification.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
and combined with diluted Taq DNA polymerase and the individual fluorescent end-labeled universal primers (see Appendix C) to yield the final reaction mixes, that are sufficient for 24 template samples. Once the above mixes are prepared, four aliquots
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
One of the major problems in DNA cycle sequencing is that when fluorescent primers (1) are used the reaction conditions are such that the nested fragment set distribution is highly dependent upon the template concentration
【分享】Taq DNA聚合酶、Pfu DNA聚合酶等酶的特点及应用
合理选择耐热DNA聚合酶是PCR成败与否的一个关键因素。选择最合适的耐热聚合酶，是进行PCR实验首先要考虑的问题。许多耐热DNA聚合酶的主要区别在于特异性、保真性、耐热性、扩增速率、扩增片段长度等几个指标。 下面是六种不同耐热聚合酶的比较： Taq：扩增效率最高的耐热DNA聚合酶，能很好的扩增6kb以下的DNA 片段。扩增碱基出错率为10-5左右。 Pfu：目前保真度最高的耐热DNA聚合酶，碱基出错率为10-6，但扩增效率低于Taq酶，一般能很好的扩增2kb以下的片段。 Taq
技术资料暂无技术资料 索取技术资料
