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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Quick-RNA Tissue/Insect Kit
- 库存:
大量
- 供应商:
简石生物
- 保存条件:
常温
HIGHLIGHTS
- High Quality: Extract intact high quality total RNA (including small/micro RNAs) from insect and arthropod specimens (mosquitoes, bees, lice, ticks, Drosophila melanogaster etc.)
- Homogenization: Ultra-high density BashingBead technology for complete disruption of tough specimen.
- Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
DESCRIPTION
The Quick-RNA Tissue/Insect Microprep Kit provides for rapid isolation of total RNA from various insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster). The Quick-RNA Tissue/Insect Microprep Kit employs ultra-high density ZR BashingBeads for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples. The Zymo-Spin IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IC Column efficiently binds total RNA. The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.
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文献和实验• 以96孔板方式,从同一个细胞或组织样本中同时纯化基因组 DNA和总RNA • 处理 96 个样本,只需不到60 分钟 • 从同一样本中纯化高品质的基因组DNA和总RNA • 96孔板方式,高效的操作流程 • 高度标准化的操作流程 • 高重复性的DNA和RNA产量 Product description The AllPrep DNA/RNA 96 Kit is well suited for high
), metatranscriptomics (community transcriptomes)(宏转录组分析), precise 3’ end mapping(3‘端精确mapping), RNA kinetics (biosynthesis and decay)(RNA动力学分析(生物合成和降解)), tissue-specific gene expression(组织特异性基因表达分析), transcriptome assembly
任何总RNA提取试剂在提取过程中无法完全避免DNA的微量残留,本公司的EASYspin系列RNA提取产品,由于采取了本公司独特的缓冲体系和选择了特殊吸附能力的吸附膜,在大多数RT-PCR 扩增过程中极其微量的DNA残留(一般电泳EB染色紫外灯下观察不可见)影响不是很大, 如果要进行严格的mRNA表达量分析如荧光定量PCR,我们建议在进行模板和引物的选择时:1) 选用跨内含子的引物,以穿过mRNA中的连接区,这样DNA就不能作为模板参与扩增反应。2) 选择基因组DNA和cDNA上扩增的产物











