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- G-Biosciences
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- GBS-786-1007
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub)
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
5x1mlcolumns
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文献和实验with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
in preparation). This explains why the GBP does not recognise unfolded or denatured GFP (e.g. on immunoblots). For immunoprecipitations of GFP fusion proteins we coupled the GBP covalently to monovalent matrices (e.g. agarose beads or magnetic particles
Qiagen kit instructions B) Low Melting Point Agarose Purification 1) Make 2% low melting point (LMP) agarose gel in TAE + Ethidium bromide. Allow gel to solidify at 5o C and store at 5o C until use. 2) Electrophorese slowly at 5o C (slow
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