- ¥486
- 江蓝纯
- P2012
- 2025年07月14日
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- 供应商：
江蓝纯
- 规格：
2ml
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P2012 | Protein A+G Agarose (Fast Flow, 进口分装) | 2ml | 486.00元 |
本Protein A+G Agarose (Fast Flow)为进口分装，主要用于免疫沉淀(Immunoprecipitation,IP)或免疫共沉淀(Co-IP)，也可以用于抗体的纯化。
Protein A+G Agarose适合于免疫沉淀所有Protein A Agarose和Protein G Agarose单独可以免疫沉淀的抗体，包括mouse IgG1, IgG2a, IgG2b, IgG3, IgA, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit IgG, rabbit and goat polyclonal Abs，以及human IgG1, IgG2, IgG3和IgG4。
Protein A和Protein G都共价交联到4% agarose beads (Fast Flow)上，2ml Protein A+G Agarose中共含有约2mg重组的Protein A+G。2 ml Protein A+G Agarose共可以结合约15mg human IgG。推荐的线性流速(Linear flow rate)为 50-
300cm/h。
Protein A+G Agarose配制在TBS溶液中，2ml中共含有0.5ml Agarose beads。
本Protein A+G Agarose如果用于常规的免疫沉淀，可以免疫沉淀100次。
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
Fast and reliable mini-prep RNA extraction from Neurospora crassa
, mycelia from plates (4 days old) and single colonies of N. crassa grown on a sorbose plate. With this protocol, from 20 to 300 mg (dry weight) of mycelium, grown in liquid media or on plates, yielded between 200 µg and 3 mg of RNA depending on the growth
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
G coupled to agarose. In the Subheading 3 there are also protocols for affinity purification using a specific ligand coupled to commercial matrices like CNBr- Sepharose 4-B and Affigel.
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