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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
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- 英文名:
G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub)
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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- 规格:
5x1mlcolumns
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文献和实验have been used to obtain enriched or purified cell populations, including flow cell sorting. Current protocols are usually time-consuming and may imply loss of short-lived RNAs, which is undesirable for expression profiling. We describe an optimized method
Comprehensive High‐Throughput Arrays for Relative Methylation (CHARM)
5 µg of high‐quality, high‐molecular‐weight, genomic DNA in 1× TE buffer ( appendix 3B ) 1× TE buffer, pH 7.4 (Quality Biological), filtered prior to use to avoid
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
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