- 询价
- T细胞与B细胞激活miRNA PCR芯片可同时检测84个与T细胞与B细胞激活相关的miRNA表达
- 上海
- 2026年04月23日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
Human T-Cell & B-Cell Activation miRNA PCR Array
- 提供商：
QIAGEN
T细胞与B细胞激活miRNA PCR芯片可同时检测84个与T细胞与B细胞激活相关的miRNA表达，miRNA列表如下：
| Product | Species | Technology | Cat. No. |
| T-Cell & B-Cell Activation miScript miRNA PCR Array | Human | miRNA Expression | MIHS-111Z |
| T-Cell & B-Cell Activation miScript miRNA PCR Array | Mouse | miRNA Expression | MIMM-111Z |
| T-Cell & B-Cell Activation miScript miRNA PCR Array | Rat | miRNA Expression | MIRN-111Z |
T-Cell Differentiation*:
Double Negative (CD4-/CD8-): let-7d-5p, let-7e-5p, miR-126-3p, miR-128, miR-146b-5p, miR-15a-5p, miR-17-5p, miR-17-3p, miR-181c-5p, miR-191-5p, miR-19a-3p, miR-199a-5p, miR-20a-5p, miR-20b-5p, miR-221-3p, miR-222-3p, miR-223-3p, miR-28-5p, miR-29c-3p, miR-30e-5p, miR-342-3p, miR-423-5p, miR-93-5p, miR-98-5p.
Double Positive (CD4+/CD8+): let-7b-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-19b-3p.
CD4+ Naïve:miR-132-3p, miR-146a-5p, miR-182-5p, miR-184, miR-25-3p, miR-326, miR-92a-3p.
CD8+ Naïve: let-7a-5p, let-7c, let-7f-5p, let-7g-5p, miR-130b-3p, miR-139-5p, miR-142-3p, miR-150-5p, miR-155-5p, miR-15a-3p, miR-16-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-30b-5p, miR-30c-5p, miR-30d-5p.
CD8+ Effector: miR-147a, miR-148a-3p, miR-18a-5p, miR-27a-3p, miR-27b-3p.
CD8+ Memory: let-7i-5p, miR-106b-5p, miR-142-5p, miR-15b-5p, miR-17-5p, miR-21-5p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-29a-3p, miR-31-5p.
B-Cell Differentiation*:
Naïve: let-7a-5p, let-7b-5p, let-7d-5p, let-7g-5p, let-7i-5p, miR-101-3p, miR-132-3p, miR-142-3p, miR-142-5p, miR-150-5p, miR-181c-5p, miR-195-5p, miR-204-5p, miR-214-3p, miR-221-3p, miR-222-3p, miR-223-3p, miR-29b-3p, miR-30e-5p, miR-331-3p, miR-92a-3p.
Germinal Center: miR-106b-5p, miR-130b-3p, miR-132-3p, miR-148a-3p, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-17-3p, miR-181a-5p, miR-181b-5p, miR-191-5p, miR-19a-3p, miR-19b-3p, miR-210, miR-23b-3p, miR-25-3p, miR-28-5p, miR-30d-5p, miR-93-5p, miR-98-5p.
Memory: miR-100-5p, miR-125b-5p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29c-3p, miR-30b-5p, miR-30c-5p, miR-34a-5p.
Differentially Expressed in Tregs: miR-100-5p, miR-125b-5p, miR-146a-5p, miR-181c-5p, miR-20b-5p, miR-21-5p, miR-31-5p, miR-335-5p, miR-365a-3p, miR-99a-5p.
T-Cell Activation:let-7e-5p, let-7g-5p, let-7i-5p, miR-106b-5p, miR-139-5p, miR-142-5p, miR-146a-5p, miR-146b-5p, miR-155-5p, miR-15a-5p, miR-15a-3p, miR-181a-5p, miR-181c-5p, miR-195-5p, miR-20b-5p, miR-214-3p, miR-223-3p, miR-23a-3p, miR-23b-3p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-342-3p, miR-346, miR-574-3p, miR-98-5p.
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文献和实验2-4 days incubation Method Prepare PBMC and resuspend the cells at 1-2x106/ml of complete RPMI. Note: This density of PBMC gives a good response. If experimental conditions require, a titration of cell densities (2-3x106/ml to 105/ml
T-Cell Activation Using mAb to CD3
in clonal expansion of T cells upregulation of activation markers on the cell surface differentiation into effector cells induction of cytotoxicity or cytokine secretion induction of apoptosis
are transmitted to the nucleus and result in 1. clonal expansion of T cells, 2. upregulation of activation markers on the cell surface, 3. differentiation into effector cells, 4. induction of cytotoxicity or cytokine secretion and 5. induction
