Cytiva ÄKTA pilot 600 pH监测器

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

and cathode buffers (see reciperecipes ) Electrophoresis apparatus: Protean II 16‐cm cell (Bio‐Rad) or SE 600 16‐cm unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers

Methods for use with the mTn-lacZ/LEU2-mutagenized library

. Screening 30, 000 transformants should give you 95% coverage of the yeast genome. To minimize double integrants, transformations should contain the lowest amount of DNA practicable. We therefore recommend that a pilot experiment be performed

Methods for use with the mTn-3xHA/lacZ-mutagenized library

. We therefore recommend that a pilot experiment be performed to determine transformation efficiency of the strain, and conditions then be scaled up as appropriate. The pilot protocol given below uses a modified version of the method of Chen et al. (1992