huh-7.5.1人肝癌细胞

Huh7.5.1/Huh7.5.1细胞系/Huh7.5.1细胞株/Huh7.5.1人肝癌细胞

Cell line name Huh-7.5.1

Synonyms Huh 7.5.1; Huh7.5.1

Accession CVCL_E049

Resource Identification Initiative To cite this cell line use: Huh-7.5.1 (RRID:CVCL_E049)

Comments Population: Japanese.

Virology: Compared to the parent Huh-7 cell line, permits improved hepatitis C virus (HCV) production when infected with strain JFH-1.

Derived from site: In situ; Liver; UBERON=UBERON_0002107.

Sequence variations

Mutation; HGNC; 6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).

Mutation; HGNC; 20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).

Mutation; HGNC; 19102; RIGI; Simple; p.Thr55Ile (c.164C>T); Zygosity=Unspecified (from parent cell line).

Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).

Mutation; HGNC; 11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).

Disease Adult hepatocellular carcinoma (NCIt: C7956)

Adult hepatocellular carcinoma (ORDO: Orphanet_210159)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Parent: CVCL_7927 (Huh-7.5)

Children:

CVCL_YU21 (Huh7.5.1-5) CVCL_C9AS (Huh-7.5.1-ACE2) CVCL_YU20 (Huh7.5.1-8)

Sex of cell Male

Age at sampling 57Y

Category Cancer cell line

Publications

PubMed=15939869; DOI=10.1073/pnas.0503596102; PMCID=PMC1166622

Zhong J., Gastaminza P., Cheng G.-F., Kapadia S., Kato T., Burton D.R., Wieland S.F., Uprichard S.L., Wakita T., Chisari F.V.

Robust hepatitis C virus infection in vitro.

Proc. Natl. Acad. Sci. U.S.A. 102:9294-9299(2005)

 

PubMed=23285155; DOI=10.1371/journal.pone.0052697; PMCID=PMC3527576

Murayama A., Sugiyama N., Yoshimura S., Ishihara-Sugano M., Masaki T., Kim S., Wakita T., Mishiro S., Kato T.

A subclone of HuH-7 with enhanced intracellular hepatitis C virus production and evasion of virus related-cell cycle arrest.

PLoS ONE 7:E52697-E52697(2012)

 

PubMed=25420655; DOI=10.7883/yoken.JJID.2014.231

Shirasago Y., Sekizuka T., Saito K., Suzuki T., Wakita T., Hanada K., Kuroda M., Abe R., Fukasawa M.

Isolation and characterization of an Huh.7.5.1-derived cell clone highly permissive to hepatitis C virus.

Jpn. J. Infect. Dis. 68:81-88(2015)