NIH/3T3

NIH/3T3/NIH/3T3细胞系/NIH/3T3细胞株/NIH/3T3小鼠成纤维细胞

Cell line name NIH 3T3

Synonyms NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3

Accession CVCL_0594

Resource Identification Initiative To cite this cell line use: NIH 3T3 (RRID:CVCL_0594)

Comments Part of: ENCODE project mouse cell lines.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Doubling time: 21.3 +- 0.5 hours (PubMed=22619183); ~20 hours (DSMZ=ACC-59).

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Miscellaneous: PubMed=23430347 has a different value for STR 4-2 (19.3) than that of NIST (19.3,20.3) due to a change in the stutter rules (personal communication of Almeida, Jamie L.).

Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (14.3) than that of NIST (15.3) due to a change in the marker motif (personal communication of Almeida, Jamie L.).

Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.

Cell type: Fibroblast; CL=CL_0000057.

PubMed=4311790; DOI=10.1128/JVI.4.5.549-553.1969; PMCID=PMC375908

Jainchill J.L., Aaronson S.A., Todaro G.J.

Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.

J. Virol. 4:549-553(1969)

 

PubMed=4087463

Iwamoto A., Masuda M., Yoshikura H.

Two NIH3T3 cell lines of different origins circulating in the world.

Jpn. J. Exp. Med. 55:129-131(1985)

 

PubMed=2463382; DOI=10.1128/JVI.63.2.753-758.1989; PMCID=PMC247747

Raptis L., Bolen J.B.

Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.

J. Virol. 63:753-758(1989)

 

PubMed=9753870

Jayashree B., Visalakshi V., Rajalakshmi K.R., Sukumaran M.S., Moni M.S., Rajkumar T., Deshmukh S.

Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.

Indian J. Biochem. Biophys. 35:108-114(1998)

 

PubMed=17284666; DOI=10.1152/physiolgenomics.00199.2006

Menger G.J., Allen G.C., Neuendorff N., Nahm S.-S., Thomas T.L., Cassone V.M., Earnest D.J.

Circadian profiling of the transcriptome in NIH/3T3 fibroblasts: comparison with rhythmic gene expression in SCN2.2 cells and the rat SCN.

Physiol. Genomics 29:280-289(2007)

 

DOI=10.3917/bhesv.191.0045

Barreiro E.W., Curras E.

Mutation carriers: led by Spaniards in U.S. laboratories in the early 80s.

Bull. Hist. Epistem. Sci. Vie 19:45-65(2012)

 

PubMed=22619183; DOI=10.1002/btpr.1564

Mancia A., Elliott J.T., Halter M., Bhadriraju K., Tona A., Spurlin T.A., Middlebrooks B.L., Baatz J.E., Warr G.W., Plant A.L.

Quantitative methods to characterize morphological properties of cell lines.

Biotechnol. Prog. 28:1069-1078(2012)

 

PubMed=23321776; DOI=10.1369/0022155413476868; PMCID=PMC3621507

Leibiger C., Kosyakova N., Mkrtchyan H., Glei M., Trifonov V.A., Liehr T.

First molecular cytogenetic high resolution characterization of the NIH 3T3 cell line by murine multicolor banding.

J. Histochem. Cytochem. 61:306-312(2013)

 

PubMed=23430347; DOI=10.1007/s10616-013-9545-7; PMCID=PMC3886540

Almeida J.L., Hill C.R., Cole K.D.

Mouse cell line authentication.

Cytotechnology 66:133-147(2014)

 

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847.1-847.11(2014)

 

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)

 

PubMed=36078083; DOI=10.3390/cells11172677; PMCID=PMC9455036

Rahimi A.M., Cai M.-F., Hoyer-Fender S.

Heterogeneity of the NIH3T3 fibroblast cell line.

Cells 11:2677.1-2677.19(2022)