MEG-01

MEG-01/MEG-01细胞系/MEG-01细胞株/MEG-01人成巨核细胞白血病细胞

Cell line name MEG-01

Synonyms Meg-01; MEG01; Meg01

Accession CVCL_0425

Resource Identification Initiative To cite this cell line use: MEG-01 (RRID:CVCL_0425)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Population: Japanese.

Characteristics: Can be induced to differentiate into megakaryocytes after application of 12-O-tetradecanoyl-13-phorbol acetate (TPA) or valproic acid (VPA) (PubMed=1742484; PubMed=30332548).

Doubling time: 36-48 hours (PubMed=2998511); ~35 hours (DSMZ=ACC-364).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Misspelling: Mego-1; Note=Occasionally.

Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.

Sequence variations

Gene fusion; HGNC; 76; ABL1 + HGNC; 1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 13 fused to ABL1 exon 2 (b2a2 transcript) (PubMed=10071072; PubMed=10576511; PubMed=15843827; PubMed=20809971; DepMap=ACH-000072).

Mutation; HGNC; 18318; ASXL1; Simple; p.Gly646Trpfs*12 (c.1934dupG); ClinVar=VCV000426927; Zygosity=Heterozygous (PubMed=26700326; DepMap=ACH-000072).

Mutation; HGNC; 11998; TP53; Simple; p.His233del (c.697_699delCAC); Zygosity=Heterozygous (Cosmic-CLP=908126; DepMap=ACH-000072).

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 1.14

Native American 0

East Asian, North 79.13

East Asian, South 17.4

South Asian 0

European, North 0

European, South 2.33

Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)

Chronic myeloid leukemia (ORDO: Orphanet_521)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_E2Q6 (HyCyte MEG-01 hJAK2_p.V617F_c.1849G>T) CVCL_3022 (MEG-01s) CVCL_VG38 (SPYMEG)

Sex of cell Male

Age at sampling 55Y

Category Cancer cell line

STR profile Source(s): ATCC=CRL-2021; Cosmic-CLP=908126; DSMZ=ACC-364; ECACC=94012401; JCRB=IFO50151

 

Markers:

Amelogenin X,Y

CSF1PO 10

D2S1338 19

D3S1358 15

D5S818 13

D7S820 11

D8S1179 14,15

D13S317 8

D16S539 9

D18S51 18,22

D19S433 14,16

D21S11 29

FGA 26

Penta D 11,13

Penta E 15

TH01 7

TPOX 8,11

vWA 16

 

Run an STR similarity search on this cell line

Publications

PubMed=2998511; DOI=10.1182/blood.V66.6.1384.1384

Ogura M., Morishima Y., Ohno R., Kato Y., Hirabayashi N., Nagura H., Saito H.

Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome.

Blood 66:1384-1392(1985)

 

PubMed=3332852; DOI=10.1016/S0950-3536(87)80037-9

Keating A.

Ph positive CML cell lines.

Baillieres Clin. Haematol. 1:1021-1029(1987)

 

PubMed=1742484; DOI=10.1182/blood.V78.12.3168.3168

Murate T., Hotta T., Tsushita K., Suzuki M., Yoshida T., Saga S., Saito H., Yoshida S.

Aphidicolin, an inhibitor of DNA replication, blocks the TPA-induced differentiation of a human megakaryoblastic cell line, MEG-O1.

Blood 78:3168-3177(1991)

 

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886

Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.

Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.

Jpn. J. Cancer Res. 89:712-718(1998)

 

PubMed=10071072; DOI=10.1016/S0145-2126(98)00171-4

Drexler H.G., MacLeod R.A.F., Uphoff C.C.

Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.

Leuk. Res. 23:207-215(1999)

 

PubMed=10576511; DOI=10.1016/S0145-2126(99)00131-9

Uphoff C.C., Habig S., Fombonne S., Matsuo Y., Drexler H.G.

ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.

Leuk. Res. 23:1055-1060(1999)

 

DOI=10.1016/B978-0-12-221970-2.50457-5

Drexler H.G.

The leukemia-lymphoma cell line factsbook.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

 

PubMed=15843827; DOI=10.1038/sj.leu.2403749

Andersson A., Eden P., Lindgren D., Nilsson J., Lassen C., Heldrup J., Fontes M., Borg A., Mitelman F., Johansson B., Hoglund M., Fioretos T.

Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.

Leukemia 19:1042-1050(2005)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

 

PubMed=20809971; DOI=10.1186/1755-8166-3-15; PMCID=PMC2944125

Virgili A., Nacheva E.

Genomic amplification of BCR/ABL1 and a region downstream of ABL1 in chronic myeloid leukaemia: a FISH mapping study of CML patients and cell lines.

Mol. Cytogenet. 3:15.1-15.12(2010)

 

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027

Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Nature 483:603-607(2012)

 

PubMed=26700326; DOI=10.1016/j.exphem.2015.11.011

Inoue D., Matsumoto M., Nagase R., Saika M., Fujino T., Nakayama K.I., Kitamura T.

Truncation mutants of ASXL1 observed in myeloid malignancies are expressed at detectable protein levels.

Exp. Hematol. 44:172-176.e1(2016)

 

PubMed=27277069; DOI=10.1160/TH15-11-0891; PMCID=PMC5080539

Wright J.R., Amisten S., Goodall A.H., Mahaut-Smith M.P.

Transcriptomic analysis of the ion channelome of human platelets and megakaryocytic cell lines.

Thromb. Haemost. 116:272-284(2016)