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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human IgG1-Fc Protein (C103S, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥7640.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥1140.0 |
蛋白名称:Human IgG1-Fc Protein (C103S, His Tag)
蛋白构建:A DNA sequence encoding the Human IGHG1 (P01857-1) (Glu99-Lys330, C103S) was expressed, with a polyhistidine tag at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Glu 99
蛋白分子量:The recombinant Human IGHG1 comprises 232 amino acids and has a predicted molecular mass of 26.08 kDa. It migrates as approximately 31.7 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:P01857-1
蛋白氨基酸序列:Glu99-Lys330(C103S)
蛋白标签:N-His
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文献和实验in the expressed sequence tag or unannotated DNA databases and were novel proteins of unknown function. Since that time, we have been using various molecular biological approaches to determine the functions of some of these proteins
to directly answer key biological questions. We believe that a similar strategy could be adopted with transgenic or knockout mouse work, particularly in cases where there is no obvious phenotype. [NextPage] Proteome mining
纯化最常用的方法之一,是亲和层析法,借助标签和配体的亲和力进行蛋白纯化。纯化的重组蛋白通常能以特异性的方式被洗脱,例如,在 Strep 标签蛋白纯化中,加入生物素(biotin)即可断开 Strep 标签与配体 Strep - Tactin 的作用,达到有效洗脱的目的。得到高纯度有活性的蛋白质应用在多种下游实验中,如研究蛋白质间相互作用等。 那么是否可以以此为基础,将标签配体亲和力加以利用,结合免疫识别特性,用温和的方法得到高纯度的目的细胞呢? 一、「画龙点睛」的表位标签—— Fab
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