异丙基-β-D-硫代半乳糖苷|IPTG *Sigma货号：I

一般描述

异丙基 β-D-硫代半乳糖苷 (IPTG) 是一种分子生物学试剂，用于诱导细菌系统中的基因表达
它模仿乳糖，与 lac 阻遏蛋白结合，导致基因激活
IPTG 对于研究基因调控、蛋白质表达和重组蛋白质生产至关重要，为细胞和分子生物学研究提供了宝贵的见解
应用
IPTG can be used for:
Inducing protein expression in GroEL and mutants of Lactobacillus reuteri [1]
Inducing protein expression in Escherichia coli [2]
IPTG is commonly used in cloning programs that require induction of β - galactosidase activity. It is used in conjunction with X-Gal or Bluo Gal for blue white spot screening of recombinant bacterial colonies, inducing the expression of the lac operon in E. coli. IPTG works by binding to the lacI repressor and changing its conformation, preventing the repressor effect of the β - galactosidase encoding gene lacZ

生化/生理作用

IPTG 是异乳糖的不可代谢类似物
它作为诱导子调节 lac 启动子的转录活性，该启动子用于在大肠杆菌中表达该蛋白质
[3]