- ￥2310 - 3870
- Sino Biological
- 北京
- 80111-R08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Rat HER3 / ErbB3 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg/100 µg
| 规格： | 50 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥3870.0 |
蛋白名称：Rat HER3 / ErbB3 Protein (His Tag)
蛋白构建：A DNA sequence encoding the rat ERBB3 (XP_017450189.1) (Met1-His641) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1.Immobilized Human NRG1 Beta 1 Fc(Cat:11609-H01H2) at 2 μg/ml (100 μl/well) can bind Rat HER3/ERBB3 His(Cat:80111-R08H) with a linear range of 0.8-4.0 ug/mL (Rountinely tested). 2.Immobilized Recombinant Rat HER3 / ErbB3 Protein (His Tag) (Cat: 80111-R08H) at 2 μg/mL (100 μL/well) can bind Anti-Human Her3 Antibody, Human IgG1, the EC50 is 12-36 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 20
蛋白分子量：The recombinant rat ERBB3 comprises 633 amino acids and predicts a molecular mass of 69.9 kDa. The apparent molecular mass of the recombinant protein is approximately 112 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：XP_017450189.1
蛋白氨基酸序列：Met1-His641
蛋白标签：C-His
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文献和实验，EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体，而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体：hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本：蜡包埋人类肺部肿瘤抗原修复缓冲液：柠檬酸盐缓冲液（左）或者 EDTA 缓冲液（右）煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后，才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然，一些研究人员也使用水浴锅，但是，因为该步骤中所选择的装置对染色存在积极或消极的影响
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
number of genes (including ERBB2 , ERBB3 , VEGF , and EPO 5-8 ). However, rational design has not always yielded functional regulators in vivo , mainly because knowledge of both regulatory areas and of endogenous factors affecting transcription factor�DNA
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