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999
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10 ml
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文献和实验a 5 mg/mL DAPI stock solution (14.3 mM for the dihydrochloride or 10.9 mM for the dilactate), dissolve the contents of one vial (10 mg) in 2 mL of deionized water (dH2 O) or dimethylformamide (DMF). The less water-soluble DAPI dihydro-chloride
), prepare fresh Anesthetic solution: dilute 80 mg/ml ketamine‐HCl/xylazine‐HCl solution (Sigma‐Aldrich) 1:10 (v/v) in sterile saline, prepare fresh
);Maleic acid buffer (0.1 M maleic acid; 0.15 M NaCl; adjust to pH 7.5 with NaOH);Blocking solution (1% blocking reagent in maleic acid buffer);Detection buffer (0.1 M Tris-HCl, pH 9.5; 0.1 M NaCl; 50 mM MgCl2);TE (10 mM Tris-HCl, pH 8.0; 1 mM EDTA);Color
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