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999
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100 assays
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文献和实验is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including
High Level Expression of Recombinant Proteins in E. coli
of mg quantities Part I. Expression Systems Expression System Components Target Protein mRNA cDNA genomic DNA Expression Vector (plasmids) pET pQE pGEX Expression Strain (E. coli ) BL21(DE3) Rosetta(DE3
Strategies to Optimize Protein Expression in E. coli
. Protein Expr. Purif 58:210‐221. Grodberg, J. and Dunn, J.J. 1988. Ompt encodes the Escherichia coli outer‐membrane protease that cleaves T7‐Rna polymerase during purification. J
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