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文献和实验Sauer:RNA Purification from E. coli
My Experience Purifying RNA from E. coli Regarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria. With Qiagen (anion exchange) or other silica-binding technologies, the smaller RNAs
is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including
Assay for ß-galactosidase in E. Coli
in the cell only when an inducer (signal) is present in the cell (e.g. ß-galactosidase or the enzymes in the tryptophan biosynthesis pathway). This procedure is designed to induce and measure the level of ß-galactosidase in E. coli. Cells grown
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