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文献和实验Liquid Chromatography‐Mass Spectrometry Analysis of DNA Polymerase Reaction Products
experimental and analytical procedures for characterizing the efficiency and fidelity of translesion DNA synthesis across various DNA damages by DNA polymerases in vitro. This procedure utilizes primer extension assays followed by LC?MS and LC?MS/MS analysis
;6.After the third wash the bound beads were eluted using elution buffer II, incubated at 65°C for 10 minutes and separated using the magnetic bead separator;7.The final ~100µl elute is then assessed for promoter specific DNA and/or RNA by PCR and RT-PCR respectively
Control Interferon-alpha treated 4 h 24 h 4 h 24 h ME-15 HuH7 CHF ME-15 HuH7 CHF ME-15 HuH7 CHF ME-15 HuH7 CHF microRNAs rated higher in HuH7 hsa-miR-122a 685 30,912 44.14*** 704 32
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