• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        DNA Polymerase Inhibition Assay (PIA) for the Detection of DrugDNA Interactions

        互联网

        611
        The authors have devised a simple method for the detection of drug–DNA interaction and sequence selectivity in vitro (1 ).The method is based on the inhibition or termination of bacterial DNA polymerase(s) when it encounters modified DNA sequences. The DNA polymerase inhibition assay (PIA) has been successfully employed to study a variety of DNA binding and bonding agents. The method was initially validated for the detection of adducts formed by sequence-selective alkylators such as adozelesm and duocarmycm A (1 ,2 ). Subsequently, the method was modified to permit detection of nonbonding intercalators and nonintercalative binding agents, such as netropsin (unpublished results). This method is relatively safe for the user, as it employs less radioisotope than is required for the detection of alkylation products by conventional postlabeling or Maxam and Gilbert chemical cleavage techniques.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序