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文献和实验A Method to Assay Inhibitors of DNA Polymerase IIIC Activity
, one of these, designated pol III, is responsible for DNA replication and growth of bacteria and, therefore, warrants consideration as a drug target. However, the pol III enzymes of Gram-positive and Gram-negative species are quite different, and the Gram-positive enzyme
to shear DNA to lengths between 200 and 1000 basepairs being sure to keep samples ice cold. 5,� Centrifuge samples (part A, step 7) for 10 minutes at 13,000 rpm at 4�C, and detected the OD260 of the lysates. 6, Sonicated lysates
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
to the ligation reaction mixture. This procedure is based on the fact that Taq polymerase has a nontemplate dependent activity that adds a single deoxyadenosine to the 3′ ends of double-stranded DNA (2 ). The Invitrogen pCR™II vector is supplied as a linearized
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