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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
SNFA-128 APC Rat IgG1,κ Isotype Control(HRPN) Rat APC SNFA-400 FITC Mouse lgG1,κ Isotype Control(MOPC-21) Mouse FITC SNFA-560 PE Mouse lgG1,κ Isotype Control(MOPC-21) Mouse PE SNFA-127 APC Mouse lgG1,κ Isotype Control
Flow Cytometric Analysis Of Bcl Family members
minutes to prevent non-specific antibody binding. Cells were washed thrice in FACS buffer, incubated with the relevant antibody or relevant isotype matched control antibodies at 4„aC for 40 minutes. This was followed by three washes in FACS buffer
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