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文献和实验Follow-Up of Natural Product Isolation
What do we mean by “follow-up”? Let us assume that the natural product just isolated is of some interest, that is to say, it may have some biological activity worthy of further examination, it may represent a novel structure
NMR Spectroscopy: Structure Elucidation of Cycloelatanene A: A Natural Product Case Study
The structure elucidation of new secondary metabolites derived from marine and terrestrial sources is frequently a challenging task. The hurdles include the ability to isolate stable secondary metabolites of sufficient purity
Site-Directed Mutagenesis Mediated by a Single Polymerase Chain Reaction Product
of less than 10%. The reason for this low efficiency may stem from the nucleotide sequence of the target DNA, which may acquire stable secondary structures, such as stem-loops. These nucleotide structures may interfere with the annealing
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