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文献和实验Freeze Squeeze PCR Product Purification from Agorose Gel
Abstract: The “Freeze squeeze” method is a cost effective and technically simple procedure to isolate and prepare PCR products for DNA sequencing. Reagents •70% ethanol •3 M sodium acetate Procedure 1.Run your PCR product of interest on a 1
A Workflow from Untargeted LC-MS Profiling to Targeted Natural Product Isolation
Liquid chromatography-mass spectrometry (LC-MS or HPLC-MS) is an extremely sensitive analytical technique that enables the detection of metabolites with a vast range of chemistries and molecular masses. Extracts from any biological starting
Isolation by Preparative High-Performance Liquid Chromatography
Preparative high-performance liquid chromatography (HPLC) has become a mainstay of natural product isolation and purification. The various modes available (e.g., normal-phase, reversed-phase, size exclusion, and ion-exchange) to date can be used
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