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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
T-Cell Activation Using mAb to CD3
monoclonal antibody; MTT assay for detection of cellular proliferation. Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol
DNA聚合酶,限制性内切酶mluI,nheI,sphI,notI均购自GIBCOBRL公司,抗CD3单克隆抗体HIT3a由本所协和科技公司提供,抗Pgp单克隆抗体PHMA02由本室制备。鼠抗E-tag抗体,HRP标记的鼠抗E-tag抗体及抗E-tag亲和层析柱均购自Pharmacia公司。FITC标记的羊抗鼠IgG多抗购自北京中山生物工程公司。其它均为国产分析纯生化试剂。(3)PCR引物P1 5’ CTA GCG CAC GCG TAC GCT GAC ATC GAG CTC ACT CAG TCT
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