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文献和实验On the Discovery of Precursor Processing
Studies of the biosynthesis of insulin in a human insulinoma beginning in 1965 provided the first evidence for a precursor of insulin, the first such prohormone to be identified. Further studies with isolated rat islets
Primary MicroRNA Processing Assay Reconstituted Using Recombinant Drosha and DGCR8
protein DiGeorge critical region 8 (DGCR8). DGCR8 has been shown to tightly bind an Fe(III) heme cofactor, which activates its pri-miRNA processing activity. Here we describe how to reconstitute pri-miRNA processing using recombinant human Drosha and DGCR8
Production and Functional Assays of Recombinant Secreted Amyloid Precursor Protein (APP) (sAPPα)
The β-amyloid precursor protein (APP) is connected to Alzheimer’s disease by both biochemistry and genetics. As the source of the major constituent of amyloid plaques, APP has been the subject of many studies of its expression and metabolism
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