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文献和实验Identification of a Mutant Kinase/ATP Analog Pair
, it is necessary to screen ATP analogs for their compatibility with the kinase mutant. This step requires an active form of the wild-type and mutant kinase, either as recombinant activated protein, or as protein that has been immunoprecipitated from transfected
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, H. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell 139:610‐622. Hudson, J.R., Jr., Dawson, E.P
Assaying Endogenous Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K) Activities
, including cell survival, cell proliferation, migration, integrin activation, and transcription. PtdIns(4,5)P 2 is at the heart of phosphoinositide signaling; its levels are controlled by enzymes that synthesize it and those that degrade
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