Recombinant Human Cyclin-Depen

Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets

kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration

Introduction of human 14-3-3 proteins

of mRNA encoding human 14-3-3 protein,a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases and an endogenous inhibitor of protein kinase C.The 1,730-nucleotide sequence of the cloned cDNA contains 191 bp of a 5'-noncoding region

Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase

with a protein kinase regulating its activity in an adenosine triphosphate-dependent manner. When native or recombinant PDE2 is immunoprecipitated from PC12 cells using an antibody to the amino terminus in a buffer containing Lubrol 12A9, protease inhibitors