Mouse IgM (isotype control),

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光

光素。由于FITC是小分子化合物，每一个抗体可标记几个FITC分子，IgM通常用小分子的荧光素标记，如FITC、Cy3/5、Texas Red等。            我们知道，FITC的绿色荧光强度肉眼观察已经很亮了，但是从上面的图可见，与Cy2相比还是偏弱，而DyLight 488则更胜一筹。DyLight是一种新型的荧光分子，与传统的荧光团如FITC、Rhodamine、Texas Red等相比，具有更强的荧光强度、更高的光学稳定性以及特异性等。同时，DyLight种类

Immunofluorescent Staining of Mouse and Rat Leukocytes

steps 6-7). We find that SAv-PE and SAv-BD Cy-Chrome™ are "brighter" than FITC conjugates and may provide even better discrimination of low-density antigens, especially in the presence of BD Fc Block™, for mouse cells. (Please see Staining Tip