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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand break repair and V(D)J recombination (1 –3 ). DNA-PK is composed of a large catalytic subunit of approx 460 kDa (DNA-PKcs) and a heterodimeric DNA targeting subunit
cAMP-dependent protein kinase (PKA) is involved in various cellular functions such as cell proliferation, gene induction, and metabolism (1 ,2 ) and its regulatory subunits have been suggested as a drug target for cancer and other diseases
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