ACTL6B基因敲除质粒

pLenti-ACTL6B-sgRNA (ACTL6B基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的ACTL6B基因敲除的质粒(L05020 pLenti-ACTL6B-sgRNA)、慢病毒(L05021 ACTL6B Knockout Lentivirus)、HEK293T细胞(L05022 ACTL6B Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L05023 ACTL6B Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L05024 ACTL6B Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

ACTL6B基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	ACTL6B	51412	BC020944	NM_016188

 

About the gene
Official Symbol	ACTL6B
Previous Symbol	ACTL6
Official Full Name	actin like 6B
Synonyms	BAF53B
Location	7q22.1
Gene Type	protein-coding gene
Uniprot ID	O94805
Pathway/Library	Epigenetic Regulators Related Genes Library
Gene Summary	The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain.