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AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
零下20℃
- 保质期：
至少一年有效
- 英文名：
MGC15959; MGC8350; H1X
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
5µg
pLenti-H1-10-sgRNA (H1-10基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
H1-10基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | H1-10 | 8971 | BC000426 | NM_006026 |
| About the gene | |
| Official Symbol | H1-10 |
| Previous Symbol | - |
| Official Full Name | H1 histone family member X |
| Synonyms | MGC15959; MGC8350; H1X |
| Location | 3q21.3 |
| Gene Type | protein_coding |
| Uniprot ID | Q92522 |
| Pathway/Library | Epigenetic Regulators Related Genes Library |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H1 family. |
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文献和实验Hu, R.; Qin, J.; Feng, W.; Song, X.; Huang, H.; Dai, C.; Zhang, B.; Chen, Y. Lysosomal zinc nanomodulation blocks macrophage pyroptosis for counteracting atherosclerosis progression. Sci. Adv. 2025, 11, eadu5278
Histone Assay Solution (10 rxns) Amount [Final] Histone Wash Buffer 320 µL - Histone H1, 2 µg/µL 18 µl 0.1 µg/µL
octamers can be purified from native sources (Thomas & Butler, 1977) or assembled from recombinant histones (Luger et al, 1999). The latter method allows the incorporation of modified histones, or histone variants. Linker histones H1 and H5 isolated
TRAF‐Mediated TNFR‐Family Signaling
. Camerini, D., Walz, G., Loenen, W.A., Borst, J., and Seed, B. 1991. The T cell activation antigen CD27 is a member of the nerve growth factor/tumor necrosis factor receptor gene family. J. Immunol. 147:3165
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