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- 技术资料
- 保存条件：
零下20℃
- 保质期：
至少一年有效
- 英文名：
H1.3; H1d; H1s-2
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
5µg
pLenti-H1-3-sgRNA (H1-3基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
H1-3基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | H1-3 | 3007 | BC104874 | NM_005320 |
| About the gene | |
| Official Symbol | H1-3 |
| Previous Symbol | H1F3 |
| Official Full Name | histone cluster 1 H1 family member d |
| Synonyms | H1.3; H1d; H1s-2 |
| Location | 6p22.2 |
| Gene Type | protein_coding |
| Uniprot ID | P16402 |
| Pathway/Library | Epigenetic Regulators Related Genes Library |
| Gene Summary | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. |
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, Tamada K, et al. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion［J］. Nat Med, 1999, 5:1365-1369.［16］ Tseng SY, Otsuji M, Gorski K, et al. B7-DC, a new dendritic cell molecule with potent
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