- ￥999
- JINGKE
- 国产
- JK17932
- 2026年09月03日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
香烟烟雾如何加速声带纤维化？CD44-透明质酸轴是关键通路Takara 秋促来袭，提取+反转录+定量套装仅 1200 元abinScience 体内级抗体 全年锁价 低至 ￥1200阿拉丁 22万+ 科研级产品现货速发，品质严控、科研无忧- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
零下20℃
- 保质期：
至少一年有效
- 英文名：
H1.3; H1d; H1s-2
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
5µg
pLenti-H1-3-sgRNA (H1-3基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
H1-3基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | H1-3 | 3007 | BC104874 | NM_005320 |
| About the gene | |
| Official Symbol | H1-3 |
| Previous Symbol | H1F3 |
| Official Full Name | histone cluster 1 H1 family member d |
| Synonyms | H1.3; H1d; H1s-2 |
| Location | 6p22.2 |
| Gene Type | protein_coding |
| Uniprot ID | P16402 |
| Pathway/Library | Epigenetic Regulators Related Genes Library |
| Gene Summary | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Hu, R.; Qin, J.; Feng, W.; Song, X.; Huang, H.; Dai, C.; Zhang, B.; Chen, Y. Lysosomal zinc nanomodulation blocks macrophage pyroptosis for counteracting atherosclerosis progression. Sci. Adv. 2025, 11, eadu5280
. Molecular mechanisms of this suppression remain elusive, however. Here, we show that a fraction of blood monocyte-derived myeloid dendritic cells (MDCs) express B7-H1, a member of the B7 family, on the cell surface.B7-H1 could be further upregulated by tumor
Furovirus Isolation and RNA Extraction
The Furovirus (fu ngus-transmitted ro d-shaped) group, whose type member is soil-borne wheat mosaic virus (SBWMV) (1 ), is a very heterogeneous group infecting a wide variety of hosts. The group consists of viruses that are naturally transmitted
, Tamada K, et al. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion［J］. Nat Med, 1999, 5:1365-1369.［16］ Tseng SY, Otsuji M, Gorski K, et al. B7-DC, a new dendritic cell molecule with potent
技术资料暂无技术资料 索取技术资料
