EP400基因敲除质粒

pLenti-EP400-sgRNA (EP400基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的EP400基因敲除的质粒(L05430 pLenti-EP400-sgRNA)、慢病毒(L05431 EP400 Knockout Lentivirus)、HEK293T细胞(L05432 EP400 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L05433 EP400 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L05434 EP400 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

EP400基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	EP400	57634	BC037208	NM_015409

 

About the gene
Official Symbol	EP400
Previous Symbol	TNRC12
Official Full Name	E1A binding protein p400
Synonyms	CAGH32; KIAA1498; P400; KIAA1818; DKFZP434I225
Location	12q24.33
Gene Type	protein-coding gene
Uniprot ID	Q96L91
Pathway/Library	Epigenetic Regulators Related Genes Library
Gene Summary	Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. EP400_HUMAN,Q96L91