NDUFA5基因敲除质粒

产品简介：

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的NDUFA5基因敲除的质粒(L01630 pLenti-NDUFA5-sgRNA)、慢病毒(L01631 NDUFA5 Knockout Lentivirus)、HEK293T细胞(L01632 NDUFA5 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L01633 NDUFA5 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L01634 NDUFA5 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

NDUFA5基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	NDUFA5	4698	BC000813	NM_005000

 

About the gene
Official Symbol	NDUFA5
Previous Symbol	-
Official Full Name	NADH:ubiquinone oxidoreductase subunit A5
Synonyms	B13; NUFM; CI-13KD-B; UQOR13; CI-13kB
Location	7q31.32
Gene Type	protein-coding gene
Uniprot ID	Q16718
Pathway/Library	Cancer Target Genes Library
Gene Summary	The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.