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文献和实验), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG
from VWR 29442-310, $24/100) Agar in omniplates seems to dry out quicker, so make sure incubator is humid. I had the Yale machine shop make metal holders that hold 3 deepwell boxes each. I attached these to the flat disc of a vertical rotating wheel
pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100) Agar in omniplates seems to dry
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