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货号:T_70311728859
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英文名称:GLASS FUNNEL 500ML FOR 78603
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
. A 50 ml culture typically yields enough RNA for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel
AFFINITY PURIFICATION OF ANTIBODIES
1. Affigel 102 (BIORAD) washed in PBS on a scintered glass funnel (3ml/column). 2. Transfer to 15ml screw cap tube in minimal volume. 3. Add 7mg SMCC (in DMF) to 2.5ml beads and mix for 2h @ RT. 4. Wash beads again in PBS. 5. Resuspend in minimal
the day of experiment.Dissolve in 100% EtOH) 1 mM Benzamidine 5 mM Iodoacetamide Whatman Paper Centrifuge Bottles Funnel Squeeze bottle Several Beakers Homogenizer (Fisher PowerGen 125) 50ml Corning tubes Ice Bucket & Ice Dounce
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