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- ProteoChem
- g4105-2ml
- 2025年07月14日
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文献和实验Immobilized pH gradient (IPG) as first dimension
Racket-shaped IPG A new sigmoidal immobilized pH gradient (IPG) was used as the first dimension. It offered high resolution and great reproducibility and allowed high protein loads. Based on our specifications, the non-linear pH gradient
the protein sample.MALDI targetMicrocentrifugeMicrocentrifuge tubes (standard and 0.7-mL)METHODFigure 1. Schematic of affinity binding of phosphopeptides to immobilized metal ion affinity columns.Preparation of the Immobilized Metal Ion Affinity Column Insert
Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion
INTRODUCTION Phosphoproteins and peptides can be bound with high specificity to immobilized metal ions such as Fe3+, Ni2+, and Ga3+. This technique can be used with either on-line or off-line MS analysis. However, elution
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