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货号:T_70311993025
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英文名称:X2 SPACERT 0,8MMX10CM
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
#______________ 1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________ X μl Nuclease free H2O to 10 μl 37℃ at least 1 hr After incubation, bring reaction volume up to 50 μl before cleaning up with spin column
. 4mls/10cm plate), add fresh trypsin/ EDTA (e.g. 1ml/10cm plate), and incubate at 37oC as appropriate to loosen cells (e.g. 1-2min for NIH3T3, 2-5min for MDCK). 3. Add 4 volumes of soybean trypsin inhibitor (1mg/ml in serum-free medium) e.g. 4mls
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