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- Thermo Fisher
- 26069-0480
- 2025年08月15日
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文献和实验Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Syringe (1-mL) (Fisher Scientific) Thermo plate (MATS) METHOD Experiment Set-Up 1. Prepare culture media (two dishes of cleavage medium with 10% SPS, and one dish of 2 mM DMAP): i. Place 10-15 drops
MBP in kinase buffer. 12.) Wash the immunoprecipitate 3x with RIPA buffer and once with kinase buffer. 13.) Add 40 μl of the reaction mixture, along with 15 μCi of gamma-32P-ATP (3000Ci/mmol, NEN) to the washed beads and mix very gently
25‐cm2 tissue culture dishes 37°C, 5% CO 2 incubator 6‐well culture plates (Nunc, Thermo Fisher
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