赛默飞世尔Thermo Fisher X2 SPACER 1

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Preparation and Analysis of Glycan Microarrays

., Smith, D.F., Wilce, M.C., Talbot, U.M., Chong, D.C., Yu, H., Huang, S., Chen, X., Varki, N.M., Varki, A., Rossjohn, J., and Beddoe, T. 2008. Incorporation of a non‐human glycan mediates human susceptibility to a bacterial toxin. Nature 456:648‐652

SDS-PAGE

and very gently squirt a 0.1% SDS overlay over the acrylamide solution (keeps oxygen away so that the top of the resolving gel polymerizes well). If you squirt this on gently enough you won't disturb the acrylamide solution, and a sharp line of refractive index